Measuring nitrogen fixation by the acetylene reduction assay (ARA): is 3 the magic ratio? - Biogeochemistry

Despite some well-documented drawbacks, the acetylene reduction assay (ARA) remains one of the most widespread methods for measuring biological nitrogen (N2) fixation (BNF) in symbiotic and free-living niches due to its low cost, simplicity, and high throughput potential. Because ARA measures a pro…